Fig. 2.

BM-DCs are activated by ZPS-conjugates. (A) Mouse BM-DCs were incubated for 20 h with ZPS and PS conjugates (10 μg/mL), CRM₁₉₇ and HSA alone (10 μg/mL), and Pam₃CSK₄ or LPS (1 μg/mL). The upregulation of CD86 and MHC class II was evaluated by flow cytometry. Data represent mean + SD of triplicates and are representative of three experiments. (B) After 20 h of incubation, cytokine and chemokine presence in the supernatants was tested through Bio-plex analysis. Data represent mean + SD of triplicates and are representative of two experiments with similar outcome.