Fig. 2.

Role of CREB-C/EBPβ cascade in normal development. (A) Epididymal fat pad weight normalized to total body weight for βΔCre mice and +/+ littermates. (B and C) Eosin hematoxylin staining on epididymal fat pad histological sections from +/+ and βΔCre mice. (D) Frequency of Mac-1⁺Gr-1^lo and Mac-1⁺Gr-1⁺ cells from +/+ and βΔCre BM determined by flow cytometry phenotyping. Data are presented as the mean ± SD (+/+ n = 3; βΔCre n = 3). (E) Macrophage colony-forming activity of BM cells were plated in methylcellulose medium containing M-CSF (10 ng/mL). Macrophage colony-forming units were scored after 8 days and are presented as average CFU-M/10³ BM cells (+/+ n = 3; βΔCre n = 3). Data are presented as the mean ± SD. (F) Frequency of prepro-B (B220⁺CD43⁺AA4.1⁺CD19⁻), pro-B (B220⁺CD43⁺AA4.1⁺CD19⁺), pre-B (B220⁺CD43⁻A4.1⁺CD19⁺), immature B (B220⁺IgM⁻), mature B (B220⁺IgM⁺), and recirculating B cells (B220⁺⁺IgM⁺) from +/+ and βΔCre BM determined by flow cytometry (+/+ n = 3; βΔCre N = 3). Data are presented as the mean ± SD. (G) Cebpb expression levels in tissues extracted from Cebpb^−/−, Cebpb^+/+ and βΔCre mice measured by real time PCR (n = 3 for each genotype). Although Cebpb mRNA levels were somewhat lower in tissues derived from βΔCre mice compared to +/+ controls, the differences were not significant. The Cebpb gene is intronless, and Cebpb^−/− mice were used to control for influence of genomic DNA contamination on the analysis. WAT, white adipose tissue.