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. 2009 Sep 24;106(41):17576–17581. doi: 10.1073/pnas.0909055106

Fig. 2.

Fig. 2.

ACC activities and lipid accumulation under lipogenic conditions of fasting and refeeding in tissues of FACC1KO mice. (A) Tissue weights expressed as percentages of body weight. WT mice and FACC1KO mice (15–16 weeks old, n = 5) were fasted for 2 days and then re-fed for 2 days with a fat-free diet to induce fat synthesis. *, P < 0.05. (B and C) The ACC and FAS activities in extracts of WAT, BAT, or livers partially purified with ammonium sulfate precipitation. *, P < 0.05; **, P < 0.0001. (D) H&E staining of adipose tissue sections show that FACC1KO mice accumulated less lipid in WAT and BAT than WT mice. The original magnification, 62×. (E) Real time PCR analyses of mRNA levels in WT mice and FACC1KO mice (n = 4).