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. 2009 Sep 25;106(41):17343–17348. doi: 10.1073/pnas.0907409106

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Fig. 1. — H₂O₂−mediated AMPK phosphorylation in endothelial cells. Shown in this figure are the results of immunoblots analyzed in endothelial cells treated with H₂O₂. (A) Representative immunoblot from a dose-response experiment analyzed in cells stimulated with the indicated concentrations of H₂O₂ for 30 min and probed with antibodies as shown; (B) pooled data from five independent experiments, analyzing the intensities corresponding to phospho-AMPK and total AMPK by quantitative chemiluminescence. (C) Representative time course experiment in BAECs treated with 200 μM H₂O₂ for the indicated times and analyzed in immunoblots probed with antibodies as shown; (D) pooled data from five independent experiments. *, P < 0.05, and **, P < 0.01 by ANOVA.