Fig. 2.

Effects of protein kinase inhibitors and NOS inhibitors on H₂O₂-induced AMPK phosphorylation. (A) Representative immunoblot analyzed in endothelial cells treated with H₂O₂ (200 μM, 30 min) after first being incubated for 30 min with inhibitors as shown: STO-609 (CaMKKβ inhibitor, 10 μg/mL); wortmannin (PI3-kinase inhibitor, 10 μM); N-nitro-L-arginine (NNA, NOS inhibitor 10 μM); or L-arginine methyl ester (L-NAME, NOS inhibitor, 100 μM). Cell lysates were subjected to immunoblotting using antibodies as shown. (B–D) Quantitative analyses of pooled data from three to five independent experiments, analyzing the phosphorylation responses for AMPK, ACC, and LKB1, respectively.