Fig. 6.

AMPK phosphorylation in tissues and cells from eNOS^−/− mice. This figure shows immunoblot analyses of liver, lung, or endothelial cells from wild-type (wt) or eNOS^−/− mice. (A) Liver, lung tissues and isolated lung endothelial cells (MLECs) from wild-type and eNOS^−/− mice were analyzed in immunoblots probed with antibodies as indicated. Shown below are pooled data from at least three experiments quantitating AMPK phosphorylation; ***, P < 0.001. (B) MLECs from wild-type or eNOS^−/− mice were treated with H₂O₂ (100 μM for 30 min). The experiment shown is a representative of five similar experiments showing that MLECs from eNOS^−/− mice have increased basal as well as H₂O₂-stimulated AMPK phosphorylation relative to MLECs from wild-type mice; pooled data are also shown,*, P < 0.05.