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. 2009 Sep 25;106(41):17343–17348. doi: 10.1073/pnas.0907409106

Fig. 7.

Fig. 7.

siRNA-mediated knockdown of AMPK and CaMKKβ impairs H2O2-induced endothelial cell tube formation. Endothelial cells were transfected with siRNA constructs targeting AMPK or CaMKKβ, and analyzed in the Matrigel tube formation assay. (A) Representative images of endothelial Matrigel tube formation in H2O2-treated endothelial cells transfected with siRNA constructs as shown. (B) Pooled data from three independent tube formation experiments, plotting the total normalized tube length relative to the total tube length as measured for untreated endothelial cells transfected with control siRNA. *, P < 0.05, **, P < 0.01.