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. 2009 Sep 14;423(Pt 1):31–39. doi: 10.1042/BJ20082398

Figure 3. Endogenous spartin can be recruited to lipid droplets.

Figure 3

(A) HeLa cells were treated with oleic acid (200 μM for 24 h) and lipid droplets were labelled by incubation with BODIPY 493/503. Cells were then fixed and labelled for spartin. A spartin signal often surrounded the lipid droplets and, where lipid droplets clustered, a honeycomb appearance was seen (see boxed area; inset shows a magnified image of the boxed area). (B) A cell in which lipid droplet formation was induced in the same way as in (A). The cell was labelled with the mitochondrial marker MitoTracker, as well as with α-spartin and BODIPY 493/503. The spartin signal surrounds many of the lipid droplets; an example is indicated by the arrowheads. The spartin label around the lipid droplets shows minimal co-localization with MitoTracker. Scale bars=10 μm.