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. Author manuscript; available in PMC: 2010 Oct 20.
Published in final edited form as: Biochemistry. 2009 Oct 20;48(41):9941–9948. doi: 10.1021/bi901425h

Figure 2.

Figure 2

Slow-onset inhibition of saporin-L1 by transition state mimics. A) Plot of lumens (RLU) versus time for saporin-L1 catalysis of A-10 with increasing inhibitor [A14 (9-DA) 2′-OMe] concentrations. B) Competitive inhibition curve fit of rate versus increasing concentrations of tetramer cyclic oxime G(9-DA)GA DNA inhibitor. Kinetics were measured after a 10 minute enzyme-inhibitor pre-incubation equilibration to achieve slow onset binding (Ki*).