Log phase cultures of WT (BWP17), Δhgt4 (CM9 and CM10), or Δhgt12 (CM64) cells were split and incubated in fresh media containing 5% galactose or 5% glycerol at 30°C for 2 hours. Total RNA was reverse transcribed and PCR amplified with primers for HGT7 (orf19.2023), QDR1 (orf19.508), AOX2 (orf19.4773), CMK1 (orf19.5911), HXK2 (orf19.542), and ACT1 (orf19.5007). Control reactions lacking reverse transcriptase yielded no products (not shown).