(A) CaSTD1 plays a role in the HGT4 pathway. Isogenic strains [HGT4 (CM87) vs. HGT4-1 (CM36) and Δstd1 (CM222) vs. STD1 (CM224)] were grown at 30°C to log phase in media containing glycerol. Cells were harvested, snap frozen, and total RNA was purified for RT-PCR analysis of HGT7 (orf19.2023) or ACT1 (orf19.5007). (B) CaSTD1 is glucose-induced. C. albicans cells (SC5314) were grown to log-phase in media containing glycerol, then incubated at 30°C for 2 hours in fresh media with glycerol (gly), or with the indicated concentrations of glucose (0 indicates no carbon source). Cells were harvested, snap frozen, and total RNA was purified for RT-PCR analysis using primers for CaSTD1 (orf19.6173) or ACT1 (orf19.5007).