Fig. 3.

The effect of PNGaseF treatment on the apparent molecular size of HsPCFT. In vitro enzymatic deglycosylation was achieved by addition of PNGaseF (10 U/mL) to 200 µg of membrane protein with incubation for 3 h at 37 °C. An equal amount of PNGaseF treated (right lane) and control (left lane) lysate (30 µg) was loaded and the blots were probed with a peptide antibody directed to the C-terminus of HsPCFT. Numbers on the left indicate the molecular sizes in the protein ladders. The blot is representative of three separate experiments.