Fig. 7.

Immunofluorescence staining of wild-type and deglycosylated HsPCFT. HeLa cells were stably transfected with cDNA of wild-type or deglycosylated HsPCFT fused with the HA epitope on either the N- or C-terminus, then exposed to Alexa Fluor 488 conjugated anti-HA monoclonal antibody. Green fluorescence indicates localization of HsPCFT, while red fluorescence indicates nucleic acids counterstained by propidium iodide. The image is representative of at least three experiments. The left panel shows cells subjected to permeabilization with Triton X-100 before exposure to antibody. The right panel shows cells that were not permeabilized.