. 2008 Mar 20;1778(6):1407–1414. doi: 10.1016/j.bbamem.2008.03.009

Table 1.

Primers for site-directed mutagenesis and PCR primers for N- and C-terminal HA-epitope insertions for wild-type and N58Q/N68Q-HsPCFT

		Primer sequence (5′ to 3′)
N58Q	Forward	GCCGACCTCGGCTACCAAGGCACCCGCCAAAGG
N58Q	Reverse	CCTTTGGCGGGTGCCTTGGTAGCCGAGGTCGGC
N68Q	Forward	AGGGGGGGCTGCAGCCAACGCAGCGCGGACCCC
N68Q	Reverse	GGGGTCCGCGCTGCGTTGGCTGCAGCCCCCCCT
N58Q/N68Q	Forward	GCCGACCTCGGCTACCAAGGCACCCGCCAAA
	Forward	GGGGGGGCTGCAGCCAACGCAGCGCGGACCCC
	Reverse	GGGGTCCGCGCTGCGTTGGCTGCAGCCCCC
	Reverse	CCTTTGGCGGGTGCCTTGGTAGCCGAGGTCGGC
N-terminal HA tag	Forward	TCAAGATCTCACCATGTACCCATACGATGTTCCA
	Forward	GATTACGCTATGGAGGGGCGCGTGAGC
	Reverse	TATAGATCTCAGGGGCTCTGGGGAAACTG
C-terminal HA tag	Forward	TATAAGCTTCACCATGGAGGGGAGCGCGAGC
	Reverse	TCATCTAGATTAAGCGTAATCTGGAACATCG
	Reverse	TATGGGTAGGGCTCTGGGGAAACTG