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Kinetics of cleavage of GRP78 by SubAB. NRK-52E cells were treated with 100 ng/ml (A) or 10 ng/ml (B) of SubAB for up to 24 (A) or 72 h (B) and subjected to Western blot analysis of C-terminal region of GRP78. The level of β-actin is shown at the bottom as a loading control.