Skip to main content
. Author manuscript; available in PMC: 2010 Jul 15.
Published in final edited form as: J Immunol. 2009 Jun 26;183(2):1480–1487. doi: 10.4049/jimmunol.0900017

FIGURE 4.

FIGURE 4

Activation of the Akt-NF-κB pathway through the UPR triggered by SubAB. A–C, NRK-52E cells were treated with SubAB and SubAA272B (A), or tunicamycin (1 μg/ml) and thapsigargin (500 nM) (B and C) for indicated time periods and subjected to Northern blot analysis of GRP78 (A and B) or Western blot analysis of phosphorylated Akt (C). D, NRK/NFκB-Luc cells were treated with tunicamycin or thapsigargin for up to 24 h and subjected to chemiluminescent assay. E, Met5A cells were transfected with pNFκB-Luc, treated with tunicamycin (Tun; 10 μg/ml) or thapsigargin (Thap; 1 μM) for 12 h and subjected to luciferase assay. Data are expressed as means ± SE, and asterisks indicate statistically significant differences (p < 0.05).