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. Author manuscript; available in PMC: 2009 Oct 16.
Published in final edited form as: Cancer Epidemiol Biomarkers Prev. 2008 Apr;17(4):995–1000. doi: 10.1158/1055-9965.EPI-07-2808

Figure 1.

Figure 1

Direct sequencing of the bisulfite PCR product from the CpG island within exon 1 of the TCF21 gene for 10 lung cancer cell lines and 2 normal lung epithelial cell lines (HBEC2 and HBEC3). Closed circles, methylated CpG sites; open circles, unmethylated CpG sites. The expression levels were quantitated using comparative Ct method. In both TCF21 and WNT4 expression analysis, means of expression values for the two HBECs were considered to have a value of 1. (expression positive, relative expression >1; expression negative, relative expression <0.50). Sequencing and expression analyses were conducted as described in Materials and Methods. Arrows, locations of primers; open box, location of the probe.