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Cbfa-1 expression is not induced by inhibition of proteoglycan sulfation. RNA from C2C12 cells after 6 days of differentiation in the absence or presence of 1 nM BMP-2 or 30 mM sodium chlorate was obtained. After reverse transcription using Oligo-dTs, Cbfa-1, myogenin and β-actin cDNAs were amplified using specific primers. The reactions were performed within the linear range for both time and RNA quantity.
