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. Author manuscript; available in PMC: 2010 Apr 1.
Published in final edited form as: Gene Ther. 2009 Jul 9;16(10):1223–1233. doi: 10.1038/gt.2009.74

Figure 3. Potency and replication of recombinant vaccinia viruses armed with human endostatin-angiostatin fusion gene and LacZ gene.

Figure 3

A, VVlacZ and VVhEA construction by insertion of the lacZ and human endostatin-angiostatin fusion genes respectively into the Not1 restriction site of VVLister; B, Potency of VVhEA and VVLacZ in human pancreatic cancer cell lines. EC50 values + SEM calculated by MTS assay 144 hours after infection with different vaccinia viruses; C and D, Replication of recombinant viruses in pancreatic cell lines infected with 1 PFU/cell VVlacZ or VVhEA. Mean viral replication ± SEM was determined by TCID50 assay.