(a,b) Movement of the T289 mutation is rescued by addition of an NLSGFP construct. Two different roots expressing the SHRpro–SHRΔT289–NLSGFP construct are shown. GFP is detected in the endodermis in both roots.
(c–f) Expression of SHRpro–SHR–NESGFP in scr-5 resulted in SHR localization similar to that for SHRpro–SHRΔVHIID [for comparison, see inset in (e)] and a reduction in SHR movement from the stele into the endodermis. The effect of the NES on SHR localization is more pronounced in scr-5 (c,e,f) than in wild-type plants (d).
M, mutant ground-tissue layer; E, endodermis; C, cortex. Arrows indicate nuclei.