Figure 4.
Transport properties of Q169N mutant PfHT1. (Left) Initial mean d-glucose uptake rates for PfHT1 and Q169N mutant (one of three independent experiments in each case; eight oocytes per concentration; mean ± SE). For PfHT1 in this experiment, Km = 1.6 mM, Vmax = 302 pmol/oocyte/h; for Q169N Km = 1.4 mM, Vmax = 650 pmol/oocyte/h. (Right) d-fructose inhibition of d-[14C]glucose uptake in PfHT1 and Q169N mutant. Uptakes (eight oocytes per concentration; mean ± SE) are expressed relative to d-glucose uptake in uncompeted oocytes.