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. Author manuscript; available in PMC: 2010 Oct 15.
Published in final edited form as: Cancer Res. 2009 Oct 6;69(20):7978–7985. doi: 10.1158/0008-5472.CAN-09-2177

FIGURE 1.

FIGURE 1

Radiation sensitivity of filamin-A proficient and deficient cells. Panel A shows the anti-filamin-A western blot for the following three pairs of filamin-A proficient and deficient cell lines. 1) M2 (FLNa−) and A7 (FLNa+): M2 is a melanoma cell line that spontaneously lost filamin-A expression, and A7 is a M2-derived cell line that was transfected with filamin-A expressing vector (29); 2) C8161 (control shRNA) and C8161-KD (FLNa-shRNA): C8161 is filamin-A positive metastatic melanoma cell line expressing a control shRNA, and C8161-KD is a C8161 sub-clone expressing a filamin-A specific shRNA; 3) MB231 (control shRNA) and MB231-KD (FLNa-shRNA): MB231 is the MDA-MB-231 breast cancer cell line expressing a control shRNA, and MB231-KD is a MDA-MB-231 sub-clone expressing a filamin-A specific shRNA. Beta-actin was used as a loading control. Colony formation assays (see Materials and Methods) were used to determine the survival fractions after irradiation. Panel B-C are the radiation survival curves for the three pairs of filamin-A proficient and deficient cells. Error bars represent the standard deviation of three independent experiments.

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