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. Author manuscript; available in PMC: 2010 Oct 15.
Published in final edited form as: Cancer Res. 2009 Oct 6;69(20):7978–7985. doi: 10.1158/0008-5472.CAN-09-2177

FIGURE 6.

FIGURE 6

Filamin-A truncations fail to complement the DSB repair defects. Several truncation mutants of filamin-A as illustrated in 6A (bottom panel) was expressed in the M2 filamin-A deficient cells. See text for full description of the truncation mutants. The cell line expressing the full length filamin-A was used as positive control, and cell line carrying an empty vector was used as negative control. Panel B shows the average number of γH2AX foci per cells shortly after irradiation (0.25 hr), and after 2 and 8 hours of repair for M2-Vector (negative control), A7 (M2 cells with full length filamin-A), and several M2 cells expressing truncated filamin-A . Panel C are Western blots used as an alterative method to measure the level of γH2AX at various times after irradiation among the same cell lines. Anti-Actin blot was used as loading control for γH2AX blot. Panel C (bottom panel) also shows the confirmed expression of filamin-A truncation mutants.