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. Author manuscript; available in PMC: 2010 Oct 16.
Published in final edited form as: Immunity. 2009 Oct 8;31(4):643–653. doi: 10.1016/j.immuni.2009.07.008

Figure 1. Retroviral-Mediated Stem-Cell Gene Transfer.

Figure 1

(A) Schematic representation of the TCRα-2A-TCRβ constructs, including the amino acid sequence of the 2A regions of Thosea asigna virus (T2A) and porcine teschovirus-1 (P2A), GSG linker, and insertion point in the MSCV-IRES-GFP vector. Conserved residues are boxed. The arrow indicates the ‘cleavage’ point between the 2A and 2B peptides. (B) Schematic representation of protocol to produce dual TCR retrogenic mice in NOD/SCID recipients. Retroviral-mediated stem cell gene transfer was performed with MSCV-IRES-GFP and MSCV-IRES-YFP constructs into separate populations of NOD/SCID bone marrow. These were then combined and injected, to produce mice with two different populations of T cells.