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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Traffic. 2009 Aug 5;10(11):1669–1684. doi: 10.1111/j.1600-0854.2009.00974.x

Figure 2. Sar1 distribution in 2 and 3 DIV neurons.

Figure 2

Rat (A & C) and mouse (B) hippocampal neurons were fixed at indicated times post plating. (A–B) Cultures were labeled with phalloidin (A1 and B1; red in A3 and B3) and antibodies to Sar1 (A2 and B2; green in A3 and B3). (C) Cultures were transfected with GFP (C2; green in C4) prior to plating and labeled with antibodies to Sar1 (C1; red in C4) and Map2 (C3; green in C4). Beginning at 3 DIV axonal Sar1 starts to associate with varicosities (arrows in C). C1-4 were made using two fields stitched together. Bars equal 10 µm.