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. Author manuscript; available in PMC: 2010 Nov 1.
Published in final edited form as: Traffic. 2009 Aug 5;10(11):1669–1684. doi: 10.1111/j.1600-0854.2009.00974.x

Figure 6. Redistribution of endogenous Sar1 after cytochalasin D induced actin destabilization.

Figure 6

(A–B) Rat hippocampal neurons were treated with DMSO only or CytD for 24 hours starting at 4 hours post plating. At 28 hours post plating cultures were fixed and labeled with phalloidin (A1 & B1) and antibodies to Sar1 (A2 & B2). (C–F) Neurons (1 DIV) were rinsed with PBS, permeabilized, and washed as described in Methods. Cells were then incubated in the presence of buffer (C), 5 µg Sar1-GTP (D), 5 µg Sar1-GTP and rat liver cytosol (E), or 5 µg Sar1-GDP and rat liver cytosol (F). At the end of the incubations, the distribution of Sar1 (C–D) and Sec13 (E–F) was determined using IF microscopy. Open arrowhead in D points to ERES in a minor neurite. The bar in F is also for C–E; bars equal 10 µm.