Table 1.
Deduced functions of orfs in the nosiheptide biosynthetic gene cluster.
| Gene | Sizea | Protein Homologb and Origin | Identity / Similarity, % | Proposed Function |
|---|---|---|---|---|
| orf1 | 324 | SCO4661 (NP_628821), from S. coelicolor A3(2) | 91/96 | elongation factor G |
| orf2 | 156 | SCO4660 (NP_628820), from S coelicolor A3(2) | 99/100 | 30S ribosomal protein S7 |
| orf3 | 123 | SCO4659 (NP_628819), from S coelicolor A3(2) | 99/100 | 30S ribosomal protein S12 |
| nosA | 151 | Francci3_4114 (YP_483191), from Frankia sp. CcI3 | 55/67 | hypothetical protein |
| nosB | 455 | Sare_3149 (YP_001537948), from Salinispora arenicola CNS-205 | 32/47 | cytochrome P450 |
| nosC | 408 | SACE_1426 (YP_001103673), From Saccharopolyspora erythraea NRRL 2338 | 39/55 | cytochrome P450-like enzyme |
| nosD | 345 | TsrK (ACN80673), from S. laurentii ATCC 31255 | 32/54 | dehydratase |
| nosE | 920 | TsrJ (ACN80672), from S. laurentii ATCC 31255 | 29/50 | dehydratase |
| nosF | 549 | TsrM (ACN80675), from S. laurentii ATCC 31255 | 29/51 | dehydrogenase |
| nosG | 656 | TsrO (ACN80677), from S. laurentii ATCC 31255 | 36/58 | cyclodehydratase |
| nosH | 654 | TsrN (ACN80676), from S. laurentii ATCC 31255 | 22/37 | hypothetical protein |
| nosI | 419 | SAMR0921 (CAJ88630), from S. ambofaciens ATCC 23877 | 29/39 | AMP-dependent acyl-CoA synthetase/ligase |
| nosJ | 79 | -- | -- | hypothetical protein |
| nosK | 264 | BCE_2449 (NP_978758), from Bacillus cereus ATCC 10987 | 29/43 | α/β fold family hydrolase/acyltransfer ase |
| nosL | 400 | ThiH (AAD48429), from Salmonella typhimurium | 26/41 | Radical S-AdoMet: Biotin and thiamine synthesis associated |
| nosM | 50 | -- | -- | NOS precursor peptide |
| nosN | 395 | Tlm Orf11 (ABL74954), from Streptoalloteichus hindustanus | 32/48 | S-AdoMet-dependent oxidase or methyl transferase |
| nosO | 370 | TsrL (ACN80674), from S. laurentii ATCC 31255 | 28/49 | hypothetical protein |
| nosP | 323 | pSLA2-L_p071(NP_851493), from S. rochei | 51/66 | pathway specific regulatory protein |
| orf4 | 1299 | SAV_4915 (NP_826092), from S. avermitilis MA-4680 | 96/98 | DNA-directed RNA polymerase subunit β′ |
| orf5 | 1181 | SAV_4914 (NP_826091), from S. avermitilis MA-4680 | 95/98 | DNA-directed RNA polymerase subunit β |
| orf6 | 129 | SCO4653 (NP_628814), from S. coelicolor A3(2) | 81/86 | 50S ribosomal protein L7/L12 |
| orf7 | 176 | SSEG_01531 (YP_002208236), from S. sviceus ATCC 29083 | 89/96 | 50S ribosomal protein L10 |
| orf8 | 272 | SCO4651 (NP_628812), from S. coelicolor A3(2) | 65/78 | lipoprotein |
| orf9 | 297 | SSEG_01533 (YP_002208238), from S. sviceus ATCC 29083 | 55/70 | lipoprotein |
| orf10 | 241 | SCO4649 (NP_628810), from S. coelicolor A3(2) | 92/97 | 50S ribosomal protein L1 |
a
Numbers are in amino acids.
b
NCBI accession numbers are given in parentheses.