Skip to main content
. Author manuscript; available in PMC: 2010 Aug 28.
Published in final edited form as: Mol Cell. 2009 Jul 30;35(4):490–501. doi: 10.1016/j.molcel.2009.07.004

Figure 4. Electron micrographs of kDNA networks and maxicircles from TbPIF2 overexpression cells.

Figure 4

(A) and (B), kDNA isolated from TbPIF2 overexpression cells one day after induction. Black arrow, long dense connection between the two sister networks; empty arrowhead, maxicircle loops with one linear branch. Bar, 500 nm. (C), EM of θ-type replicating maxicircles. After in vivo trioxsalen cross-linking, kDNA was isolated, decatenated by topoisomerase IV and analyzed by EM. Arrows indicate branch points. Bar, 500 nm.