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. 1998 May 26;95(11):6212–6216. doi: 10.1073/pnas.95.11.6212

Figure 3.

Figure 3

Detection of the qid74 gene in different species. (A) Southern blot analysis of genomic DNA, digested with BamHI (B) and XmaI (X). (B) PCR amplification of the ORF of qid74, using the 5′ (Q74up) and 3′ (Q74lo2) ends as sense and antisense primers (see Materials and Methods). (C) Variation of tandemly repeating sequences analysis. A single-copy sequence oligonucleotide (Q74up) was used as sense primer and, as antisense, a oligonucleotide (Q74lo1) was used that would hybridize with different melting temperatures to a number of nonidentical repeats. In this condition, 10 bands, which include from the ORF of repeat C to the ORF of repeat L, were expected in T. harzianum 2413. Hypocrea jecorina showed the same pattern of T. reesei (data not shown). Lanes: M, molecular weight marker; 1, T. harzianum 2413; 2, T. viride; 3, T. virens; 4, T. harzianum IMI; 5, T. longibrachiatum; 6, T. koningii; 7, T. reesei; 8, Hypocrea jecorina; T, bands expected in T. harzianum 2413; EB, expected bands in T. harzianum 2413.