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. Author manuscript; available in PMC: 2010 Jul 10.
Published in final edited form as: J Mol Biol. 2009 May 13;390(2):319–338. doi: 10.1016/j.jmb.2009.05.005

Fig. 6.

Fig. 6

Sedimentation velocity analysis of PKR binding to TARwt monomer. (a) Plot of normalized g(s*) distributions for 1 µM TARwt monomer (red), and 1 µM TARwt monomer plus 0.5 µM PKR (green), 1 µM PKR (blue) or 4 µM PKR (black). The distributions are normalized by area under the curve. (b) Global analysis of the sedimentation difference curves. Scans within each dataset were subtracted in pairs to remove time-invariant background and fit to a 1:1 binding model using SEDANAL.63 Top panels show data points and the solid lines represent fitting results using the parameters presented in Table 2. Bottom panels show residuals for each fit. Only every 4th difference curve is shown for clarity purpose. Measurements were performed in AU200 buffer at 20 °C and 40 000 RPM using absorbance detection at 260 nm.