Table 1.
RNA hydrodynamic parameters and molecular weights.
| RNA | Mexperimental (kDa)a | Mpredicted (kDa) | sb | RMSc |
|---|---|---|---|---|
| Monomers | ||||
| TARwt | 21.2 (20.9, 22.3) | 18.3 | 3.22 | 0.00678 |
| A34U | 21.4 (20.6, 22.0) | 18.3 | 3.19 | 0.00604 |
| U37A | 21.8 (20.7, 22.6) | 18.3 | 3.20 | 0.00634 |
| scTAR | 18.0 (17.3, 18.6) | 16.7 | 2.98 | 0.00689 |
| Dimers | ||||
| TARwt | 34.7 (31.1,39.0) | 36.6 | 4.25 | 0.00721 |
| A34U:U37A | 39.9 (38.7,41.2) | 36.6 | 4.31 | 0.00708 |
| scTAR | 32.0 (31.3,32.7) | 33.4 | 4.07 | 0.00618 |
Parameters obtained by global nonlinear least-squares analysis of sedimentation velocity experiments.
The values in parentheses represent the 95% joint confidence intervals. All data in this table were collected in AU200 buffer.
a
Experimental molecular weights obtained by fitting to a single ideal species model and the Svedberg equation.
b
Uncorrected sedimentation coefficient (Svedbergs).
c
RMS deviation of the fit in absorbance units.