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. Author manuscript; available in PMC: 2010 Jul 10.
Published in final edited form as: J Mol Biol. 2009 May 13;390(2):319–338. doi: 10.1016/j.jmb.2009.05.005

Table 2.

PKR–RNA interaction parameters

RNA Modela Kd1 (nM) Kd2 (nM) s (RP)b s (RP2)b RMSc
Monomers
TARwt 1 667
(589,752)		 - 5.10
(5.04,5.16)		 - 0.00699
A34U 1 752
(641,900)		 - 5.01
(4.93,5.09)		 - 0.00729
U37A 1 926
(746,1150)		 - 4.92
(4.84,5.00)		 - 0.00637
scTAR 1 188
(148, 236)		 - 4.97
(4.88,5.07)		 - 0.00595
Dimers
TARwt 2 404
(257,648)		 2760
(1170,4540)		 5.51
(5.02,5.95)		 7.70
(6.97,8.60)		 0.00794
A34U:U37A 2 331
(226,490)		 1470
(910,2430)		 5.29
(5.13,5.47)		 7.18
(6.86,7.66)		 0.00581
A34U:U37A 3 980 2.58×106 6.75 11.85 0.00785

Parameters obtained by global nonlinear least-squares analysis of sedimentation velocity experiments.

The values in parentheses represent the 95% joint confidence intervals. All data in this table were collected with wild type PKR in AU200 buffer.

a

Model 1 is R+P=RP; Model 2 is R+P=RP, RP+P=RP2; Model 3 is R+P=RP; 2RP=(RP)2.

b

Uncorrected sedimentation coefficient (Svedbergs).

c

RMS deviation of the fit in absorbance units.