Fig. 3.

GFP–PspA and PspG–GFP traffic between the cell poles. (i) Inverted epifluorescence images of E. coli MG1655ΔpspA/GFP–PspA and MG1655ΔpspG/PspG–GFP cells. Polar GFP fusion proteins are indicated with grey arrows, lateral complexes with black arrows. (ii) Inverted time-lapsed epifluorescence images of MG1655ΔpspG/PspG–GFP cells. The movement of one of the mobile lateral complexes is indicated in each image by a black arrow. For clarification, schematic figures were drawn to illustrate the movement of the complex. The spot which represents the mobile fluorescent complex was shaded from black to light grey to indicate increasing time (i.e. black represents t = 0 s and white with grey outline represents t = 5.12 s). Composite figures were made to demonstrate the movement of the complex over time (a–e, f–j, a–j). All images were taken using a Nikon TE-2000 inverted optical microscope with a time resolution of 80 ms/frame. (For original images see Supporting information and the supplementary video clip.)