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. 2009 Jul 22;24(11):2767–2777. doi: 10.1093/humrep/dep247

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© The Author 2009. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org

The online version of this article has been published under an open access model. Users are entitled to use, reproduce, disseminate, or display the open access version of this article for non-commercial purposes provided that: the original authorship is properly and fully attributed: the Journal and Oxford University Press are attributed as the original place of publication with the correct citation details given: if an article is subsequently reproduced or disseminated not in its entirety but only in part or as a derivative word this must be clearly indicated. For commercial re-use, please contact journals.permissions@oxfordjournals.org

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Expression of MECA-79 and sulfotransferase (GlcNAc-GST2) in epithelial cells isolated from endometrial biopsies.

(A) and (D) Protein extracts of epithelial cells isolated from biopsies obtained from fertile (F, n = 6), endometriosis (Endom or Endo, n = 6), PCOS (n = 6) and UI (n = 6) patients were analyzed for the expression of MECA-79 (A) and GlcNAc-GST2 (D) as described in Materials and Methods. Protein levels were normalized to GAPDH protein levels (A). In fertile (F) women MECA-79 as well as GlcNAc-GST2 was strongly expressed compared with endometriosis (endom.), PCOS and UI. Representative blots are showed. (B) Densitometric analysis of each MECA-79 component was performed using the Quantity One software (Bio-Rad). Values are band density normalized to GAPDH and are expressed as intensity per square milimiters (INT*mm²). Values are expressed as median (⊗) and inter-quartile range (box and whisker). Fertile (white box), PCOS (grey box), UI (Dark grey box), Endometriosis (Endom, hatched box). (C) Densitometric analysis of the area covering all MECA-79 components was perfomed using the Quantity One software (Bio-Rad). Values are band density normalized to GAPDH and expressed as Intensity per square milimiters (INT*mm²). Values as expressed as median (⊗) and inter-quartile range (box and whisker). Fertile (white box), PCOS (grey box), UI (Dark grey box), Endometriosis (Endom, hatched box). (E) Densitometry analysis of the immunoblots GlcNAc-GST2 levels was performed using the Quantity One software (Bio-Rad). Values obtained are expressed as band density compared with fertile patients and are expressed as percent. Values are median (⊗) and inter-quartile range (box and whisker). Fertile (white box), PCOS (grey box), UI (Dark grey box), Endometriosis (Endom, hatched box). Statistical analysis of the samples (B, C and E) was performed using the Kruskal–Wallis test to determine significance between groups followed by a Mann–Whitney test to compare specific group pairs. *P ≤ 0.05 and **P ≤ 0.01 are considered significant.