Figure 2.

Derivation of the hESC lines VUB26_QUATRO (a–h) and VUB27_PATRU (i–p) by plating and outgrowth of a single blastomere-derived morula (days after OPU).

VUB26_QUATRO: The blastomere-derived morula (a) produced an outgrowth (arrow) represented by a compact group of cells (b, c). Four days after plating, flattened TE-like cells were visible (d) but they did not survive the following days (e). By Day 16, hESC-like cells were visible for the first time (f). They subsequently developed into a hESC colony-like structure (g) that was mechanically passaged and cultured further (h). VUB27_PATRU: the blastomere-derived morula attached to the fibroblast feeder layer at Day 5 (j). Starting from Day 6 (k), a colony-like structure developed and continued to expand (l–o). It was mechanically passaged on Day 23 and cultured further (p).