Table II.
The primer sequences and conditions of the PCRs for the gene expression studies.
| gene | Forward primer | Reverse primer | Annealing temperature (°C) | Product size (bp) |
|---|---|---|---|---|
| NANOG | CAGAAGGCCTCAGCACCTAC | CTGTTCCAGGCCTGATTGTT | 55 | 216 |
| POU5F1 | GACAACAATGAGAACCTTCAGGAGA | TTCTGGCGCCGGTTACAGAACCA | 55 | 218 |
| HPRT | GCCGGCTCCGTTATGGCG | AGCCCCCCTTGAGCACACAGA | 55 | 226 |
| REX-1 | GCGTACGCAAATTAAAGTCCAGA | CAGCATCCTAAACAGCTCGCAGAAT | 58 | 306 |
| SOX-2 | CCCCCGGCGGCAATAGCA | TCGGCGCCGGGGAGATACAT | 58 | 448 |
| LIN 28 | AGTAAGCTGCACATGGAAGG | ATTGTGGCTCAATTCTGTGC | 58 | 420 |
| NPM1 | TGGTGCAAAGGATGAGTTGC | GTCATCATCTTCATCAGCAGC | 58 | 343 |
| GDF3 | AGACTTATGCTACGTAAAGGAGCT | CTTTGATGGCAGACAGGTTAAAGT | 58 | 150 |
| DNMT3B | TTGTAGCCATGAAGGTTGGC | TGTGTAGTGCACAGGAAAGC | 59 | 351 |
| AFP | GCTGGATTGTCTGCAGGATGGGGAA | TCCCCTGAAGAAAATTGGTTAAAAT | 55 | 216 |