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. Author manuscript; available in PMC: 2009 Oct 18.
Published in final edited form as: Microbiology (Reading). 2009 Mar;155(Pt 3):979–988. doi: 10.1099/mic.0.024539-0

Fig. 4.

Fig. 4

Analysis of the preparative steps for the OMPs of C. jejuni strain 81–176 using Sarkosyl. (a) Silver-stained SDS-PAGE gel of the preparative steps. Lanes: 1, whole-cell lysate (10 μl); 2, lysate following French pressure lysis of whole cells (10 μl); 3, ultracentrifuge supernatant from first wash with 10 mM HEPES, pH 7.4 (10 μl); 4, ultracentrifuge supernatant from second wash with 10 mM HEPES, pH 7.4 (10 μl); 5, supernatant following Sarkosyl incubation (10 μl); 6, supernatant following final wash with 10 mM HEPES, pH 7.4 (10 μl); 7, Sarkosyl-insoluble proteins (OMPs) (5 μg). Molecular masses are indicated on the right (in kDa). (b–d) Immunoblots using the cytoplasmic marker anti-Cj0355 (b), the inner-membrane marker anti-CetA (c) and the outer-membrane marker anti-MOMP (d). All antibodies were used at 1: 1000 dilution.