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Gel Filtration Profile of Cytoslic SKase Activity from Young and Old Rat Hearts. The cytosolic fractions isolated from 6-month-old (-●-) and 27-month-old (-○-) rat hearts were chromatographed on a Sephacryl S200 gel filtration column. The fractions were assayed for SKase activity as described in Methods. The profile is representative of four separate experiments. The identification of the SK1 and SK2 peaks was described previously (Vessey et al. 2007).
