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. 2009 Jul-Aug;2(3):166–171. doi: 10.4161/oxim.2.3.8608

Figure 3.

Figure 3

Representative mass chromatograms measuring MEHP detected in day 1 (A) and day 42 (B) supernatants collected from stored packed red blood cell (PRBC) units (N = 10). MEHP (RT = 7.4 min, [M + H+] = 279.16) was detected using prepared standards (1–100 µM). For each run, 10 µL of sample was injected onto a YMC-Pack Protein-RP HPLC column (Waters, Milford, MA) heated to 50°C. A 20 minute linear gradient from 10 to 40% solvent B using water/0.1% trifluoroacetic acid (solvent A) and acetonitrile/0.1% trifluoroacetic acid (solvent B) was utilized with a flow rate of 1 mL/min.