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. 2009 Jul-Aug;2(3):166–171. doi: 10.4161/oxim.2.3.8608

Figure 4.

Figure 4

Total (A) DEHP and (B) MEHP in day 1 and day 42 supernatants collected from stored packed red blood cell (PRBC) units (N = 10). DEHP and MEHP levels were quantitated by liquid chromatography/mass spectrometry (LCMS) analysis. For each run, 10 µL of sample was injected onto a YMC-Pack Protein-RP HPLC column (Waters, Milford, MA) heated to 50°C. A 20 minute linear gradient from 10 to 40% solvent B using water/0.1% trifluoroacetic acid (solvent A) and acetonitrile/0.1% trifluoroacetic acid (solvent B) was utilized with a flow rate of 1 mL/min. Data are expressed in micromolar (µM) concentrations. An asterisk (*) designates significance where p < 0.05 compared to day 1 supernatants (student t-test). Significant increases in DEHP and MEHP release from the PRBC bags were observed in the day 42 samples compared with the day 1 samples.