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. 2009 Jul-Aug;2(3):166–171. doi: 10.4161/oxim.2.3.8608

Figure 5.

Figure 5

Effect of DEHP and MEHP on IL-8 release in HUVEC. HUVEC were grown to near confluency (>90%) in 48-well plates in endothelial growth medium-2 (EGM-2) containing 2% fetal calf serum, hydrocortisone, human fibroblast growth factor B, vascular endothelial growth factor, recombinant insulin-like growth factor-1, ascorbate, human epithelial growth factor, gentamycin and heparin. HUVEC were treated with media only (control), DEHP, or MEHP for 24 hours in duplicate. An IL-8 ELISA was performed on the 24-hour supernatants. Data are expressed as % IL-8 release increase versus control wells. An asterisk (*) designates significance where p < 0.05 compared to control wells (student t-test). At 10, 100 and 1,000 nM MEHP, a significant increase in IL-8 release was observed. At 10 and 100 nM DEHP, a significant increase in IL-8 release was observed. This indicates the potential pro-inflammatory capability of older units of stored packed red blood cells (PRBC).