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Effects of glucosamine on endothelial superoxide anion generation. (A) Dihydroethidium (DHE) fluorescence staining shows that glucosamine itself (0.5 mmol/L, 5 days) induced superoxide generation in HUVECs and did not affect the effect of TNFα (1 ng/ml, 10 minutes, n = 3). (B) Quantification of the above results. *p < 0.05 and **p < 0.01 vs. control.
