Figure 3.
Best fits of the 1DNC’ and 1DNCα couplings by single value decomposition to the crystal structure of non-myristoylated mARF1-GTP-Δ17. Fit of the backbone amide 1DNH couplings measured from an interleaved pair of TROSY-HSQC experiments and an HNCO-based 3D J-NH experiment is provided as a reference. The alignment tensor parameters are indicated, including the Euler angles (by z-y-z convention) α, β and γ for transforming the given molecular frame into the principle axis frame, the alignment principle order parameter Szz and the asymmetry parameter η (η = (Sxx−Syy)/Szz ).