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. 2009 Nov 6;5(11):e1000712. doi: 10.1371/journal.pgen.1000712

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Tokuhiro et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Epididymal sperm were cultured in TYH medium for 1 h. The OAZ-t-null heads were easily separated and aggregated. The nuclei were stained with PI after PFA fixation. Analysis of OAZ-t-null sperm by optical microscopy.