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| Genotype | 1 hour | 3 hours | 6 hours | Calcium Ionophore* | Living sperm**(%) |
| +/+ | 39.7±3.2 | 48.8±2.1 | 63.0±13.7 | 95.3±2.5 | 60.66±0.35 |
| +/− | 30.0±5.3 | 39.7±2.1 | 50.0±7.5 | 81.3±3.8 | 60.87±0.67 |
| −/− | 11.3±0.6 | 28.3±2.5 | 33.7±5.7 | 34.3±3.8 | 61.55±0.95 |
Values are means±SD; n = 3 assays.
*: Calcium ionophore A23187 was added to the medium after 3 hours.
**: Propidium iodide-negative sperm was measured by flow cytometer after 6 hours.
