Females expressing the experimental, synthetic Y chromosomes have retarded development, but no other reductions in fitness. (a) The fraction of females emerging vs. time since eggs were laid, for females expressing a synthetic Y from experimental EA (NG = 29 = 107, NG = 30 = 143) and EB (NG = 29 = 130, NG = 30 = 153), or control CA′ (NG = 29 = 114, NG = 30 = 141) and CB′ (NG = 29 = 126, NG = 30 = 148) males. The fraction of females emerging by day 10 is significantly lower in the experimental lines (PG = 29 = 0.0163, PG = 30 = 0.0161, directed Student’s t tests, df = 2). (b) The total number of adult offspring (measures net female fitness independent of development time) produced by EAB or CAB females (red-eyed offspring) in competition with tester-females (pink-eyed offspring). There is no significant difference between lines (P = 0.625, directed χ2 contingency test).