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. Author manuscript; available in PMC: 2009 Oct 19.
Published in final edited form as: Laser Photon Rev. 2009 Feb 1;3(1-2):159–179. doi: 10.1002/lpor.200810031

Figure 5.

Figure 5

(online color at: www.lpr-journal.org) Modeling absorption and fluorescence interactions. A Absorption imaging detects light that has not been absorbed. Each photon scattering event maintains some directionality. B Fluorescence imaging detects only light which has been emitted from a fluorophore after absorption of an incident photon. This interaction results in isotropic emission, and a change in wavelength of the light. C For discrete fluorescence perturbations, light detected at the fluorescence emission wavelength can only have originated from within the fluorescent area. D For fluorescence perturbations in a fluorescent background, the fractional change in fluorescence can be measured.