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. Author manuscript; available in PMC: 2009 Oct 19.
Published in final edited form as: J Cell Physiol. 2009 Apr;219(1):143–151. doi: 10.1002/jcp.21661

Fig. 2. Effect of PTH on phospho-p44/42 in COBs from Fgf2+/+ and Fgf2−/− mice.

Fig. 2

A, Expression levels of phospho-p44/42 in COBs were analyzed by Western blotting as described under methods. COBs from Fgf2+/+ and Fgf2−/− were treated with PTH 10−9M from 15 min to 24h. Proteins (5 µg) from each sample were subjected to SDS–PAGE, transferred to PVDF membrane and probed with a polyclonal anti-phospho-p44/42 antibody. Filters were stripped and reprobed with a polyclonal anti-p44/42 antibody to show equal loading. B, Statistical analysis from pool of three different experiments demonstrated that PTH increased phospho-p44/42 proteins only in Fgf2+/+ COBs. (Mean± SD *p <0.05)