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. Author manuscript; available in PMC: 2009 Oct 19.
Published in final edited form as: J Cell Physiol. 2009 Apr;219(1):143–151. doi: 10.1002/jcp.21661

Fig. 5. A, Effect of PTH and FGF-2 on Runx-2 labeling in Fgf2−/− COB cells transfected with the Fgf2 or vector construct.

Fig. 5

COBs from Fgf2−/− mice were transfected with a FGF-2 pMg1cla construct containing the enhanced GFP (eGFP) gene used to visualize expression of protein mediated by CMV vectors. Column a: micrographs, obtained by fluorescent microscopy show GFP labelling in cells transfected with only vector construct. Column b: micrographs show labelling for Runx-2 in cells transfected with only vector construct (red: Texas Red staining). Column c: micrographs showing GFP labelling in cells transfected with Fgf2 coding sequence construct. Column d: micrographs showing labelling for Runx-2 in cells transfected with Fgf2 coding sequence construct (red:Texas Red staining). Bar, 50 µm. B, Effect of PTH and FGF-2 on phospho-CREB labeling in Fgf2−/− COB cells transfected with the Fgf2 or vector construct. COBs from Fgf2−/− mice were transduced with a FGF-2 pMg1cla construct containing the enhanced GFP (eGFP) gene used to visualize expression of protein mediated by CMV vectors. Column a: micrographs, obtained by a fluorescent microscopy show GFP labeling in cells trasfected with only vector. Column b: micrographs show labelling for CREB in cells transfected with only vector (red: Texas Red staining). Column c: micrographs show GFP labeling in cells transfected with Fgf2 construct. Column d: micrographs showing labelling for phospho-CREBs in cells transfected with Fgf2 construct (red:Texas Red staining). Bar, 50 µm.