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. 2009 Oct 28;4(10):e7650. doi: 10.1371/journal.pone.0007650

Figure 2. Specificity of anterior endoderm as inducer of cardiac fate.

Figure 2

(A) anterior endoderm induces markers of cardiovascular cell types: blood (Scl, Globin), smooth muscle (SM actin), macrophages (Lurp-1) and endothelium (Msr), but not skeletal muscle (MLC1) or neural tissue (N-tubulin). (B) Quantification of RT-PCR shown in A. (C) Late tadpole-stage AC/AE explants express markers of ventricular myocardium Irx4 and MLC1v and of the proepicardium, Tbx18. (D) animal cap/vegetal pole conjugates (Nieuwkoop sandwiches) express skeletal muscle and neural, but not cardiac markers, in contrast to AC/AE conjugates.